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numb overexpression plasmids  (OriGene)
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OriGene numb overexpression plasmids
Fig. 5 Cell proliferation, migration, and invasion affected by <t>NUMB</t> modulation in melanoma cells. a Cell proliferation assay was performed using RealTime-Glo MT Cell Viability Assay (Promega) in NUMB-overexpressing (NUMB-wild type; S413A-non-phosphorylatable; S413D-phosphomimetic) melanoma cells till 72 h. b Cell migration was analyzed by wound healing assay in NUMB-modulated melanoma cells at 0, 24 and 48 h post-wound creation. c Cell invasion was analyzed by Matrigel invasion chambers using NUMB-modulated melanoma cells at 24 h. The quantitative data are presented as mean ± SEM with statistical significance compared to empty vector (pCMV6) control (**p < 0.01; ***p < 0.001; ****p < 0.0001) or compared to NUMB <t>overexpression</t> (#p < 0.05; ###p < 0.001; ####p < 0.0001 in ≥2 biological replicates with ≥3 technical replicates and 2-3 images per replicate. Scale Bar=400 µm. Statistical significance was determined using one- or two-way ANOVA followed by Fisher’s LSD tests.
Numb Overexpression Plasmids, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/numb+overexpression+plasmids/NUMB+(NM_001005743)+Human+Tagged+ORF+Clone/pm38184733-214-5-11
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Fig. 5 Cell proliferation, migration, and invasion affected by NUMB modulation in melanoma cells. a Cell proliferation assay was performed using RealTime-Glo MT Cell Viability Assay (Promega) in NUMB-overexpressing (NUMB-wild type; S413A-non-phosphorylatable; S413D-phosphomimetic) melanoma cells till 72 h. b Cell migration was analyzed by wound healing assay in NUMB-modulated melanoma cells at 0, 24 and 48 h post-wound creation. c Cell invasion was analyzed by Matrigel invasion chambers using NUMB-modulated melanoma cells at 24 h. The quantitative data are presented as mean ± SEM with statistical significance compared to empty vector (pCMV6) control (**p < 0.01; ***p < 0.001; ****p < 0.0001) or compared to NUMB overexpression (#p < 0.05; ###p < 0.001; ####p < 0.0001 in ≥2 biological replicates with ≥3 technical replicates and 2-3 images per replicate. Scale Bar=400 µm. Statistical significance was determined using one- or two-way ANOVA followed by Fisher’s LSD tests.

Journal: NPJ precision oncology

Article Title: Role of PLK1/NUMB/NOTCH in epithelial-mesenchymal transition in human melanoma.

doi: 10.1038/s41698-023-00493-7

Figure Lengend Snippet: Fig. 5 Cell proliferation, migration, and invasion affected by NUMB modulation in melanoma cells. a Cell proliferation assay was performed using RealTime-Glo MT Cell Viability Assay (Promega) in NUMB-overexpressing (NUMB-wild type; S413A-non-phosphorylatable; S413D-phosphomimetic) melanoma cells till 72 h. b Cell migration was analyzed by wound healing assay in NUMB-modulated melanoma cells at 0, 24 and 48 h post-wound creation. c Cell invasion was analyzed by Matrigel invasion chambers using NUMB-modulated melanoma cells at 24 h. The quantitative data are presented as mean ± SEM with statistical significance compared to empty vector (pCMV6) control (**p < 0.01; ***p < 0.001; ****p < 0.0001) or compared to NUMB overexpression (#p < 0.05; ###p < 0.001; ####p < 0.0001 in ≥2 biological replicates with ≥3 technical replicates and 2-3 images per replicate. Scale Bar=400 µm. Statistical significance was determined using one- or two-way ANOVA followed by Fisher’s LSD tests.

Article Snippet: Empty vector (pCMV6-Entry #PS100001) and NUMB overexpression plasmids were purchased from Origene (WT NUMB #RC220960 with custom mutated plasmids containing S413A and S413D mutations).

Techniques: Migration, Proliferation Assay, Viability Assay, Wound Healing Assay, Plasmid Preparation, Control, Over Expression